The motorised chair, used by the physicist after he was paralysed with motor neuron disease, sold for £296 750 in a Christie's online auction. It had been expected to fetch £10,000 to £15,000.
A collection of his medals and awards, including honours from the Royal Astronomical Society, sold for £296,750, with the entire collection achieving £1,384,625.
In addition to Hawking's personal documents and items, an original production script for Stephen Hawking's final appearance on The Simpsons-in which he was portrayed as a disc jockey with gold chains and a Kangol hat-also sold 6,250 pounds ($8,157).
Christie's said before the nine-day online auction that the items represented the "ultimate triumph of scientific brilliance over adversity" and "Stephen Hawking was a huge personality worldwide".
In addition to chairs were sold dissertation Hawking in 1965, "properties of expanding Universes".More news: Vietnam to host F1 race from 2020 in Hanoi
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The scientist at the University of Cambridge died on March 14 at 76 years of age after suffering a neurological disease that kept him prostrate to a wheelchair for more than 50 years.
The second most expensive item in the collection is the beloved physicist's former wheelchair, which raised $390,000. It featured pieces belonging to other celebrated thinkers including Albert Einstein and Charles Darwin.
Cambridge Professor Stephen Hawking has provided a posthumous windfall for two charities.
According to the Guardian, the esteemed physicist's daughter Lucy Hawking said the auction was an opportunity for his admirers to "acquire a memento of our father's extraordinary life in the shape of a small selection of evocative and fascinating items".