Several hours after the initial tweet, Spartak published another post with midfielder Fernando, speaking in Russian, saying that there was "no racism at Spartak, we are friends and a family".
Federation Internationale de Football Association president Gianni Infantino insisted racism will not be tolerated during the tournament and said World Cup referees had been given the power to abandon matches should they deem it necessary.
Russian Federation and it's troubled racism slurs are in the news again after Premier League side Spartak Moscow caused controversy by posting a racially insensitive tweet from their warm weather training camp.
The post, which has subsequently been deleted, was released on the club's official and verified Twitter account on Saturday and included emoji symbols of chocolate bars and a sunshine in the headline.
Anti-discriminatory body Kick It Out added: "This social media post from the official account of Spartak Moscow only continues to highlight the prejudices towards black people in Russian Federation". Spartak earlier said Dzhikiya would take control of its Twitter feed for the day.
It later posted a video in which Fernando says: "At Spartak there is no racism".More news: DeMarco Murray ruled out vs. Patriots
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Anti-discrimination group the FARE Network, which investigates racism issues for European soccer's governing body UEFA, criticized Spartak.
A later video showed Dzhikiya and the three Brazilians arm-in-arm, with the Russian global saying: "There is no racism at Spartak, we are all a big family".
"This shows a shocking level of ignorance".
The incident comes just six months before Russian Federation hosts the World Cup, and is the second alleged incidence of racism in which the club have been involved this season.
Leonid Mironov, the club's youth-team captain, was last month charged with racist behaviour towards Liverpool's Rhian Brewster during a UEFA Youth League match.